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Figure 1: (a) GTG banded processed metaphase spread from LPS stimulated bone marrow cultures. (b) GTG banded karyogram spread from LPS-stimulated bone marrow cultures showing t(9;15), marked with small arrows, and both normal chromosome 22. Chromosome 17 appears normal. (c) Inverted DAPI FISH image of the same matched metaphase as in Figure 1a using BCR-ABL1 probe from Vysis, Abbott, showing BCR-ABL1 fusion signal located on chromosome 9 instead of chromosome 22. Red signal (ABL1) on normal chromosome 9, fusion signal on involved chromosome 9 and two green signals (BCR) on normal chromosome 22. (d) FISH done on LPS stimulated bone marrow cultures using TP53 probe from Vysis, Abbott, and showing 2 green 1red signal pattern indicating deletion of the TP53 gene in the interphases only

Figure 1: (a) GTG banded processed metaphase spread from LPS stimulated bone marrow cultures. (b) GTG banded karyogram spread from LPS-stimulated bone marrow cultures showing t(9;15), marked with small arrows, and both normal chromosome 22. Chromosome 17 appears normal. (c) Inverted DAPI FISH image of the same matched metaphase as in Figure 1a using BCR-ABL1 probe from Vysis, Abbott, showing BCR-ABL1 fusion signal located on chromosome 9 instead of chromosome 22. Red signal (ABL1) on normal chromosome 9, fusion signal on involved chromosome 9 and two green signals (BCR) on normal chromosome 22. (d) FISH done on LPS stimulated bone marrow cultures using TP53 probe from Vysis, Abbott, and showing 2 green 1red signal pattern indicating deletion of the <i>TP53</i> gene in the interphases only