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Figure 1: Mixing studies and VWF multimer analysis in a patient with AVWS VWF multimers electrophoresed in 1.6% agarose in the presence of sodium dodecyl sulfate and detected by reaction with 125l labeled affinity-purified antibody. Incubation of the normal pooled plasma with patients plasma done at 37°C at different time intervals (0 hour, 1 hour, 2 hours and 4 hours) prior to loading in the gel. Disappearance of low molecular weight multimers of normal pooled plasma on incubation with patients plasma suggests the presence of anti-VWF antibodies

Figure 1: Mixing studies and VWF multimer analysis in a patient with AVWS VWF multimers electrophoresed in 1.6% agarose in the presence of sodium dodecyl sulfate and detected by reaction with 125l labeled affinity-purified antibody. Incubation of the normal pooled plasma with patients plasma done at 37°C at different time intervals (0 hour, 1 hour, 2 hours and 4 hours) prior to loading in the gel. Disappearance of low molecular weight multimers of normal pooled plasma on incubation with patients plasma suggests the presence of anti-VWF antibodies