Journal of Postgraduate Medicine
 Open access journal indexed with Index Medicus & EMBASE  
     Home | Subscribe | Feedback  

ORIGINAL ARTICLE
[View FULLTEXT] [Download PDF
 
Year : 2017  |  Volume : 63  |  Issue : 3  |  Page : 151-156  

Evaluation of the association between a single-nucleotide polymorphism of bone morphogenetic proteins 5 gene and risk of knee osteoarthritis

AC Sharma1, RN Srivastava1, SR Srivastava1, A Agrahari1, A Singh1, D Parmar2 
1 Department of Orthopaedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India
2 Developmental Toxicology Division, IITR, Lucknow, Uttar Pradesh, India

Correspondence Address:
R N Srivastava
Department of Orthopaedic Surgery, King George's Medical University, Lucknow, Uttar Pradesh
India

Background: Osteoarthritis (OA) is a chronic degenerative disorder probably affected by both genetic and environmental causes. Bone morphogenetic proteins (BMPs) are bone-derived factors that can induce new bone formation. Single-nucleotide polymorphisms (SNPs) of BMP5 gene alters the transcriptional activity of the BMP5 promoter that has been involved in OA susceptibility. This case–control study investigated the association of rs1470527 and rs9382564 SNP of BMP5 gene with susceptibility to knee OA (KOA). Materials and Methods: A total of 499 cases with radiographic KOA and 458 age- and sex-matched healthy controls were enrolled. Venous blood samples were obtained from all the cases as well as controls for polymerase chain reaction-restriction fragment length polymorphism. Results: The genotype distribution for rs1470527 and rs9382564 SNP was significantly different in cases and controls (P < 0.0001). Within both the SNPs of BMP5 gene, genotype CT and TT were significantly (P < 0.0001) associated with KOA as compared to the CC genotype. T allele of both the studied SNP was significantly associated with KOA (P < 0.0001). The allele frequencies of rs1470527 were 0.56(T) and 0.44(C) in cases and 0.33(T) and 0.67(C) in controls and in rs9382564 were 0.57(C) and 0.43(T) in cases and 0.71(C) and 0.29(T) in controls. Further in relation with clinical severity of OA, we observed signification association of TT genotype with both visual analog scale (P < 0.0001) and Western Ontario and McMaster Universities score (P < 0.05). Conclusion: Our results indicate significant association of rs1470527 and rs9382564 polymorphism of BMP5 gene with KOA.


How to cite this article:
Sharma A C, Srivastava R N, Srivastava S R, Agrahari A, Singh A, Parmar D. Evaluation of the association between a single-nucleotide polymorphism of bone morphogenetic proteins 5 gene and risk of knee osteoarthritis.J Postgrad Med 2017;63:151-156


How to cite this URL:
Sharma A C, Srivastava R N, Srivastava S R, Agrahari A, Singh A, Parmar D. Evaluation of the association between a single-nucleotide polymorphism of bone morphogenetic proteins 5 gene and risk of knee osteoarthritis. J Postgrad Med [serial online] 2017 [cited 2022 May 17 ];63:151-156
Available from: https://www.jpgmonline.com/article.asp?issn=0022-3859;year=2017;volume=63;issue=3;spage=151;epage=156;aulast=Sharma;type=0


 
Tuesday, May 17, 2022
 Site Map | Home | Contact Us | Feedback | Copyright  and disclaimer