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Haplotypes frequencies of CYP2B6 in Malaysia
N Musa1, MI Zulkafli1, N Talib1, N Mohamad1, H Fauzi2, R Ismail1
1 Institute for Research in Molecular Medicine (INFORMM), School of Medicine, University Sains Malaysia, Malaysia
2 Department of Pharmacy, University Teknologi MARA, Malaysia
Correspondence Address:
R Ismail
Institute for Research in Molecular Medicine (INFORMM), School of Medicine, University Sains Malaysia
Malaysia
Background: Drugs with complex pharmacology are used in the management of drug use disorder (DUD) and HIV/AIDS in Malaysia and in parts of South-East Asia. Their multiethnic populations suggest complexity due to the genetic polymorphism, such as CYP2B6 that metabolizes methadone and anti-retroviral. Aims: Our aim was to explore the genetic polymorphism of CYP2B6 among Malays, Chinese, Indians, and opiate-dependent individuals in Malaysia. Settings and Design: The study utilized DNA from our previous studies on CYPs and new recruitments from opiate-dependent individuals. Materials and Methods: For the new recruitment, after obtaining consent and baseline demography, 5 ml blood was obtained from patients attending methadone maintenance therapy (MMT) Clinics. Genomic DNA was extracted using standard methods. 10 nucleotide changes associated with CYP2B6*10, CYP2B6*2, CYP2B6*17, CYP2B6*11, CYP2B6*8, CYP2B6*14, CYP2B6*9, CYP2B6*4, CYP2B6*6, CYP2B6*27, and CYP2B6*20 were determined using multiplex nested allele-specific PCR. Statistical Analysis: Descriptive statistics were used to summarize demographic data. Differences in allele frequencies between populations were tested using Chi-squared test and were corrected using the Bonferroni test. Results: CYP2B6 polymorphism in Malaysia is variable with trends that suggest an ethnic difference. Reduced activity CYP2B6*6 occurred in 13% to 26% among Malays, Chinese, Indians and opiate-dependent individuals. Another «SQ»reduced activity«SQ», CYP2B6*2 allele, was found at much lower percentages in the groups. Conclusions: The relative commonness of reduced-activity CYP2B6 alleles in our study called for attention in terms of dosage requirements for MMT and ARV in Malaysia. It also implored follow-up association studies to determine its relevance and consequences in personalized medicine for drug use disorder and HIV/AIDS.
How to cite this article:
Musa N, Zulkafli M I, Talib N, Mohamad N, Fauzi H, Ismail R. Haplotypes frequencies of CYP2B6 in Malaysia.J Postgrad Med 2012;58:235-241
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How to cite this URL:
Musa N, Zulkafli M I, Talib N, Mohamad N, Fauzi H, Ismail R. Haplotypes frequencies of CYP2B6 in Malaysia. J Postgrad Med [serial online] 2012 [cited 2023 Oct 3 ];58:235-241
Available from: https://www.jpgmonline.com/article.asp?issn=0022-3859;year=2012;volume=58;issue=4;spage=235;epage=241;aulast=Musa;type=0 |
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